Effectiveness of purple led for inactivation of Bacillus subtilis and Escherichia coli bacteria in in vitro sterilizers

Bacteria are inactivated using a technique called photodynamic inactivation, which combines light with photosensitizer the right spectrum. The objective of this study is to ascertain e­ciency purple LEDs for photoinactivating Bacillus subtilis and Escherichia coli bacteria as well ideal LED exposure energy density. This involves exposing radiation. Two elements variation used during irradiation. first illumination at distances 3 cm, 6 9 12 cm. second changing amount radiation 30, 60, 90, 120 minutes. Total Plate Count (TPC) method was count number colonies. Statistical tests were utilized in data analysis, namely One Way Anova test (analysis variance). results indicated that 395 nm irradiation caused decrease Log CFU/mL bacteria. Inactivation showed higher mortality percentage than Changes other also death highest 98.5% 94.3% position C an distance cm density 524 J/cm 2 time shows increased increasing doses greatest Gram-positive .